EXTH-35. ANTI-TUMOR AROMATHERAPY FOR MALIGNANT BRAIN TUMORS USING VOLATILE COMPONENTS OF HIBA ESSENTIAL OIL

Abstract INTRODUCTION Glioblastoma is a brain tumor with very poor prognosis that highly resistant to treatment. Hiba (Thujopsis dolabrata) has been used as building material because of its insect repellent effect and refreshing fragrance. In this study, we analyzed the antitumor effects thujopsen, which contained in essential oil hiba, investigated aroma therapy.Methods RESULTS We anti-tumor hiba on glioblastoma cells (U251, T98G), gastric cancer (MKN45), breast (MCF7). The varied depending carcinoma, strong inhibition cell proliferation observed MKN45, U251, T98G cells. Hinokitiol reported have effects, isolated identified new factor, thujopsene, by liquid chromatography analysis. assays, thujopsen inhibited more rapidly potently than hinokitiol. Comparing reach two compounds, hinokitiol showed broader thujopsen. expression caspase-3 caspase-9 was increased DARTS analysis PKM2 an intracellular binding protein DISCUSSION present study revealed induces apoptosis via caspase-9. Furthermore, unlike hinokitiol, suggested exhibit mediated PKM2. CONCLUSION Thujopsen low molecular weight compound easily evaporates into air, it expected be applied therapy using "aroma".